Modification of Spectroscopic Method for Determination of L-arginine in Different Tissue extracts

A simple technique was established for the specific modification of guanidine groups in different extracts of tissues involving reaction with ninhydrin. The extent of the reaction is simply determined by spectrophotometric analysis. The method compared with HPLC. This modified method made easily determined in micro-quantities of L-arginine, which were operating conditions for the successful determination of arginine were optimized. The results shed the light on reduction of interference substance with and given accuracy as compared with HPLC analytical method with acceptable recovery percent as well as this method given the amount of free type of arginine based on rapid reaction of amino group of L-arginine and compartmentalization was neglected the bound type profile. Finally the procedure was display high reproducibility and sensitivity of different biological tissues extracts, further studies need to explore the pharmacokinetic using of this method for given their applicability.