The Effect of Colloidal Silica Nanoparticles on the Activity of α-Amylase

The effect of silica nanoparticles on the activity of α-amylase is determinations using isothermal titration calorimetry. It was found that the immobilized enzyme activity increased as evidenced by the stability parameters recovered from the extended solvation theory. The stability indexes of the immobilized α-amylase was less than of the free enzyme, thereby the activity of the enzyme was increased as a result of its interaction with silica nanoparticles. The present report shows that silica nanoparticles are activator of α-amylase, as the complexes of silica+ α-amylase are less stable than the free enzyme.