Detection, Isolation, Purification, and Characterization of Mannose Binding Lectin (ManBL) from Patients with Different Kidney Diseases and Healthy Individuals

The present study was designed to investigate the presence of mannose binding lectin (ManBL) in the sera and tissue samples of patients with tumoral (benign & malignant) kidney in addition to patients with non tumoral kidney affection, in order to establish the possibility of using serum and tissue ManBL test for diagnosis and epidemiological testing of kidney cancers. Participants of the present study were 96 patients at the age range 12-80 years; they were attending by different hospitals in Najaf (basically) and Karbala, and 46 healthy individuals within the same age range of the studying patients groups. Specific hemagglutination activity levels were revealed a significant increased (p<0.001) in the sera and tissues of the patients with the malignant tumors specimens in comparison to that of pathological controls and healthy individuals. Serum and tissue hemagglutination activity levels were significantly higher (p<0.001) in patients with metastatic disease compared with patients with localized tumors. While, the levels of the hemagglutination activity were approximate at patients with the end malignant kidney tumors stages. Upon electrophoresis of the study samples, the results reflected presence of changes in some proteins and glycoproteins bands presents in these samples. Using hydrophobic affinity chromatography, ManBLs were purified from sera of malignant, benign kidney tumors and non tumoral kidney diseases in addition to healthy individuals groups. The purified lectins were found to be glycoprotein with 77.45 KD as an approximate molecular weight and its sugar content was equal to13.5%. The maximum concentration of purified ManBL was found at patients with malignant kidney tumors.The results revealed that the highest hemagglutination activity of purified ManBL occurred with O⁺ RBCs, at pH 7.4, and 37˚C. ManBL (regardless of its origin) lost the hemagglutination activity, completely, in the presence of EDTA. A result that indicates that the present purified the human ManBLs is a calcium dependent type.